Course Description
This course provides the basic and advanced principles of genetic engineering in yeast and mammalian cells for the overproduction of a protein of interest. Students will use classical and novel strategies to establish a stable Chinese hamster ovary (CHO) cell line based on the industrially relevant strain, DG44 (DHFR system). Students will also generate a yeast expression system based on Pichia pastoris for the production of the same protein. The generation of two different cell lines for the overproduction of the same protein of interest should provide students with an appreciation of each system in terms of cost, speed, productivity and product quality.
Non-Std Tm
Through Fall 2026
Spring 8W2 2025
Instructors
Meeting Patterns
Classes Start:
February 27, 2025
Classes End:
April 22, 2025
Location:
00112 BTEC
Class Days:
M F
Class Start Time:
1:40pm
Class End Time:
4:25pm
Class Type:
Laboratory
Credits:
0.00
Restrictions:
Corequisite: BEC 425/525 or Prerequisite: BIT 410/510 or MB 351 or MB 352 or MB 354
