Course Description
This course is an introduction to fundamental molecular biology techniques, applied to generate bacterial cell lines for the production of recombinant proteins. Course material provides a comprehensive description of an expression system, with emphasis on the central dogma of molecular biology, detailed gene structure, vector components and bacterial host cell characteristics. Different genetic, physiologic and growth condition aspects are included to ensure the overproduction of a functional protein of interest. This comprises different molecular approaches for gene cloning, bacterial selection/screening and regulation of genetic expression. The course provides hands-on experience during laboratory sessions, where students isolate a gene of interest, clone the gene into an expression vector, transform bacteria, select for positive clones, grow recombinant cells, and induce the production of the protein of interest. Techniques such as SDS-PAGE, Western blot, and ELISA are used for the detection and quantification of the active recombinant protein.
Non-Std Tm
Through Fall 2026
Spring 8W1 2025
Instructors
Meeting Patterns
Classes Start:
January 6, 2025
Classes End:
February 26, 2025
Location:
00105 BTEC
Class Days:
F
Class Start Time:
12:50pm
Class End Time:
5:50pm
Class Type:
Laboratory
Credits:
0.00
Restrictions:
Prerequisite: BIO 183 or equivalent