Course Description
Introduction to molecular biology and protein chemistry. Theory behind laboratory techniques and overview of cloning strategies starting from nucleic acid or protein sequence data. Laboratory sessions involve subcloning, preparation of competent cells, transformation, screening recombinant DNA by colony hybridization and PCR, SDS-PAGE of recombinant protein, affinity purification, and western blots.
Spring 2025
Instructors
Meeting Patterns
Classes Start:
January 6, 2025
Classes End:
April 22, 2025
Location:
06117 Jordan Hall
Class Days:
W
Class Start Time:
12:50pm
Class End Time:
2:40pm
Class Type:
Lecture
Credits:
4.00
Restrictions:
Prerequisite: BIO 183 or ZO/BIO 160 and CH 223 or CH 227 with a C- or better