Course Description
This course provides the basic and advanced principles of genetic engineering in yeast and mammalian cells for the overproduction of a protein of interest. Students will use classical and novel strategies to establish a stable Chinese hamster ovary (CHO) cell line based on the industrially relevant strain, DG44 (DHFR system). Students will also generate a yeast expression system based on Pichia pastoris for the production of the same protein. The generation of two different cell lines for the overproduction of the same protein of interest should provide students with an appreciation of each system in terms of cost, speed, productivity and product quality.
Non-Std Tm
Through Fall 2024
Fall 8W2 2024
Instructors
Meeting Patterns
Classes Start:
October 10, 2024
Classes End:
December 3, 2024
Location:
00129 BTEC
Class Days:
M F
Class Start Time:
12:50pm
Class End Time:
1:40pm
Class Type:
Lecture
Credits:
2.00
Restrictions:
Corequisite: BEC 425/525 or Prerequisite: BIT 410/510 or MB 351 or MB 352 or MB 354
